Possible reason:The glass slide is not cleaned before the sample is made

Recommended solution:Wash the glass slide with absolute ethanol

Possible reason:Washing is insufficient after hybridization

Recommended solution:Ensure that the PH value and temperature of the washing liquid are correct, remove the cover glass, and wash repeatedly

Possible reason:The filter group is used improperly

Recommended solution:Replace the appropriate filter group to observe to reduce the background light

Possible reason:The washing temperature is too low

Recommended solution:When the glass slide is washed, ensure that the temperature of solution reaches the temperature required in the LBP’s manual 

Possible reason:The sample is not well fixed

Recommended solution:Try to replace with other paraffin blocks for treatment, or recommend pathologists to communicate with clinicians in time for fixation time and effect

Possible reason:The washing liquid is used for a long time or stored incorrectly

Recommended solution:Ensure the use frequency of washing liquid, replace the washing liquid in time, and store the washing liquid according to the requirements of LBP’s manual